Speed and accuracy
BioNotus has experience with a diversity of in vitro and in silico tools to select your most promising drug candidates rapidly and accurately.
Bioanalytical support for your in vivo discovery PK studies
BioNotus develops sensitive LC-MS methods to support in vivo plasma PK profiling. Typical turnaround time 5-7 working days. Ask for our competitive pricing with or without method transfer.
Subcellular fractions
including microsomes and S9 fractions are versatile in vitro tools for swift elucidation of metabolic stability in multiple species including human
Caco-2 monolayers
This legendary in vitro model remains the gold standard for determination of apparent permeability (Papp) coefficients, assessment of transport polarity and/or transporter involvement
Suspended hepatocytes
... represent one of the most complete in vitro tools to support ranking based on metabolic half-life and to allow prediction of intrinsic and in vivo hepatic clearance (CLint).
Determination of fraction unbound (fu) in plasma
BioNotus applies high-throughput equilibrium diaslysis as the gold standard method for plasma protein binding. Ultrafiltration can be applied on customer request.
Drug transporter phenotyping
Membrane vesicles and transporter-transfected cell lines provide valuable insights in the role of specific membrane transporters in the disposition of your compounds
Aqueous solubility
Solubility measurements in various buffer systemms (pHs) are key for predicting oral bioavailability of your test compounds.
Drug drug interactions
Most in vitro tools mentions can also be applied in customized drug drug interaction tools. Early prediction of DDI potential is key to a reliable drug discovery process.
Cytotoxicity assessments
Cytotoxicity assessments in various cell lines are pivotal to prioritize compounds and to interpret potency values for efficacy
Custom hepatotoxicity assays
BioNotus is specialised in predicting hepatotoxicity liability of your compounds, inlcuding DILI and drug-induced cholestasis